Cellulolytic enzymes production from isolated fungus from enviromental samples of Cuatro Cienegas, Coahuila

Producción de enzimas celulolíticas a partir de hongos aislados de muestras ambientales de Cuatro Ciénegas, Coahuila

Dafne Hebe Ramírez-Lozano1 , Alma Soria-Ortiz2 , Baltazar Gutiérrez-Rodríguez2 , Raúl Cuauhtémoc Baptista-Rosas1 , Yolanda Garza-García1 , José Gerardo Gaona-Lozano1* Departamento de Biotecnología, Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila. Saltillo, Coahuila, CP 25280.

* jggaona@uadec.edu.mx

ABSTRACT

Cellulose is the most abundant polymer in nature and currently is used as a promising energy source. Their biological transformation is made possible by the action of microorganisms able to produce different types of cellulases. The objective of this work focuses on the reactivation of fungal strains isolated from environmental samples of Cuatro Cienegas, Coahuila and evaluation their cellulolytic activity to determine their potential to degrade cellulose in order to produce these enzymes for biotechnological application. To explore the fungus diversity were realized macroscopic and microscopic studies of 24 strains from the Biotechnology department, the cellulolytic activity of this microorganism was evaluated qualitatively through hydrolysis halo using congo-red and carboximethylcellulose (CMC) as the substrate, seven fungal strains presented this activity and were selected. The selected strains identification was made by molecular techniques according with the 18S SSU rRNA nucleotidic sequence analysis, resulting the genera Penicillium, Alternaria and Phaeosphaeria. Keywords: cellulases, Cuatro Cienegas, fungi.

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